Evidence 2 Significant branch support prior to the duplication event: nodes 2, 4, 10, 11, 12, 16, 17, 18, 19, 23, 24, 22, 26, 29, 31, 33, 34, 39. Significant branch support subsequent to the duplication event (only relevant if orthologous groups include more than one member): nodes 12, 17, 23, 31, 34, 35, 36, 38, 39. Alignment: mafft (G-INS-i, JTT200) Data model: manual (man2) Evol model: JTT + G RAxML: Best-scoring ML tree, model parameters optimized, branch support values from 1000 rapid bootstraps.