Evidence 3 Significant branch support prior to the duplication event: nodes 1, 2, 3, 4, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 18, 19, 20, 22, 32, 34, 35, 37, 39. Significant branch support subsequent to the duplication event (only relevant if orthologous groups include more than one member): nodes 1, 2, 4, 5, 6, 8, 12, 17, 21, 22, 23, 38. Alignment: mafft (L-INS-i, BLOSUM62) Data model: manual (man1) Evol model: LG +G(8) PhyML: BioNJ initial tree, search strategy Best of NNI and SPR, SH aLRT branch support values are indicated.